Hi everyone, Has anyone experienced a problem with sigmas cc/mount solution.
I liberally cover my section with it to form a protective seal., But with my current bottle it seems that when it dries it re-exposes the tissue and does not form a protective layer. I end up having to reapply it. The 2nd apply seems to get me the protective layer that I want, but I am concerned that drying/exposure from the first application will damage the staining that I have. I don't want my post staining slides to be exposed to air for a length of time between hematoxylin and cover slipping. This is a fairly recent problem and I am wondering if I just have a bad batch of cc/mount. CAT# C9368-30 ML Lot# MKBR27838V I called Sigma and they are sending me a new bottle from a different lot. But they haven't experienced this problem before with cc/mount. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
