Hey, The previous supervisor switched the Histo lab to CBG's Formula 83 with no problems as our xylene substitute. In fact, we even distil it and use the distilled in both our staining and processor. The only con is that it is not as effective in removing coverslips from slides. Unfortunately, I'm not sure about cost effective but our lab tries to balance cost-effective with people-friendly. The cyto lab uses Shandon's due to special requirements on their equipment with no problems.
"https://www.cbgtechnologies.com/hap-free-solvents.aspx"; Angela D. Hall, BA, HT(ASCP)CM Histology Department American Esoteric Laboratories www.ael-east.com ________________________________ Tel +423 586 3240 ext 1019 or 1041 Fax +423 714 2001 This message and any files transmitted with it may contain privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message, you must not disseminate, copy or take any action in reliance on it. If you have received this message in error, please notify the sender immediately. -----Original Message----- From: Bharti Parihar [mailto:bhartolog...@gmail.com] Sent: Wednesday, October 21, 2015 6:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene and Formalin substitutes Hello everyone. I'm interested in starting a conversation at my workplace regarding substitutes for xylene, and if there are any substitutes for formalin. If anyone out there is using any can they please give me feedback regarding the following questions, 1. Name of substitutes? 2. Pros and Cons? 3. Any quality issues when performing H&E, IHC or Special Stains? 4. Is it cost effective? 5. Does it require different dehydrants other than alcohol? 6. If formalin is still used as fixative, what xylene substitute works best with formalin? I appreciate any feedback! Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
