I saw a Histonet post that Peggy Wenk had made concerning the recipe for 
alcoholic formalin for processing. She noted that you use the solution 
unbuffered.  How do you control acid hematin pigment in such a solution? 
We use a commercial alcoholic formalin “concentrate” that is diluted with 
alcohol and water prior to use.  It is supposedly buffered.  We have great 
problems with acid hematin.  Do you have any suggestions?  For preprocessing 
fixation, we make our own buffered formalin from scratch or from a concentrate 
and get biopsy specimens from veterinarians fixed in who-knows-what. 
Thank you very much, 
DW 
  
Card
  

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