> From: Angela Yepes <angelapatric...@gmail.com> > Date: November 12, 2015 at 6:05:57 PM EST > To: histonet@lists.utsouthwestern.edu > Subject: Mohs sections > > >> From: Angela Yepes <angelapatric...@gmail.com> >> Date: November 12, 2015 at 4:15:50 PM EST >> To: histonet-requ...@lists.utsouthwestern.edu >> Subject: Mohs sections >> Hello everyone: >> While cutting Sections for Mohs surgery I noticed the tissue detaching from >> the O.C.T. Embedding medium and subsequent rolling of the tissue. >> To correct the problem the chuck was rotated to allow cutting from the >> opposite side (epidermis side). This maneuver did not correct the problem. I >> tried to unroll the edges with stiff brush which seemed to correct the >> problem, but it did not. I only found out the sections were still rolling >> up after the staining. >> The cryostat temperature was -23C and the sections thickness was -14 microns >> Please see pictures. >> Please help >> >> >> >> >> Sent from my iPhone >> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
