Dear all, I did a Masson's Trichrome Stain in frozen liver samples with cancer and it turns quite well to differentiate the collagen in the tumour from the normal liver but I would also like to see better the nuclei detail and have a less intense pink. Here is the protocol that I am using with the Sigma kit:
(Fixation with 4%PFA for 10 minutes) 1- Bouin's Solution at room temperature over-night; 2- Wash in tap water until remove yellow color; 3- Weigert's Hematoxilin for 5 min; 4- Was in tap water for 5 min; 5- Rinse in deionized water; 6- Biebrich Scarlet-Acid Fucshin for 5 minutes; 7- Rinse in deionized water; 8- Phosphomolybdic Acid for 5 minutes; 9- Aniline blue solution for 5 minutes; 10-Acetic acid 1% 1'30''; 11-Rinse in water, dehydrate, clear and mount. All the solution are made at the moment of the staining. I have experience with the staining but only in paraffin embedded sections and I would like to know if there is any step to be careful or change with frozen sections. Anyone can help me please? Thank you, Camila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
