Our latest CAP survey was returned today and although there are no major issues 
one possible improvement area is evident.   On all of the biopsies the area of 
"fixation/processing" was not rated as excellent.  The suggested possible 
reasons were:   fixation incomplete, nuclear bubbling artifact, tissue poorly 
processed.   The survey also said that many of the samples sent in throughout 
the country had similar  issues in the fixation/processing area most likely 
because of the rapid turnaround times and shortened processing times.   I am 
trying to be proactive here and see if we can adjust some times to improve the 
processing quality even though we have not had any complaints from the 
pathologists.   Of course we all know that other artifacts caused prior to the 
specimen arriving in the lab can also have an effect on the quality of the H&E 
slides.   Our fixation times should not be a factor so I have to conclude that 
maybe the rest of our processing times need to be adjusted.   Another
  factor that we have is that we use blue sponges for almost all of our 
tissues.  Our largest number of specimens are GI biopsies.    If possible can 
anyone share with me their rapid processing schedules or simply the approximate 
times they have for each dehydration or clearing step. We do run a larger 
overnight tissue run on any biopsy or tissue that we feel is too large for the 
"rapid run".    I am hesitant to run the biopsies routinely on the longer 
programs becase of over dehydration, etc. even though we do use an alcohol 
blend.

Any suggestions or similar experiences please share.    Again our pathologists 
say everything looks great so I don't want to change much.

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com>



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