Interesting comments. The use of this sample depends on if the antigen survives fixation and paraffin embedding. About 45-55% of our work is done with immunofluorescence on FFPE slides. we quench the autofluorescence with a CuSO4 treatment.
James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -----Original Message----- From: Simmons, Christopher via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 8:10 AM To: Rene J Buesa <rjbu...@yahoo.com> Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] DIF on paraffin embedded tissue Useless sample Sorry Sent from my iPhone > On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > No matter WHO to tell you to do WHAT, for IF purposes, that FFPE > tissue is USELESS.René > > > On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > > We have a tissue sample that was processed and paraffin embedded. We > URGENTLY need to recover the tissue and perform Immunofluorescence on > the sample. > Does anyone have a procedure. HELP > > madeathri...@pastnashville.com > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
