I need some advice on processing mouse brain. We have an outside lab using our automatic processor and embedding equipment and has been having issues section their mouse brain. I was informed that the sections are extremely brittle and rolling but mostly just the paraffin and not the tissue itself. They are seeing the brain tissue separate from the paraffin once it is the water bath.
Had better results when placing a soaked gauze on the surface of the block instead of chilling or soaking block to prevent swelling of tissue. Here is the protocol they are using for harvesting and processing: 1. Tissue perfused to 4% PFA at time of sacrifice Brain is cut in half at this point and separated 2. Tissue placed in cassette in 10% NBF for 24hrs 3. Then placed in 70% ETOH until able to process (usually the next day) 4. Processing Schedule (all done with vacuum) 30min 70% ETOH 30min 80% ETOH 30min 90% ETOH 30min 100% ETOH 30min 100% ETOH 30min 100% ETOH 30min xylene 30min xylen 5min empty tank to remove excess xylene 30min paraffin 30min paraffin I am not a histotech so any advice you can give me would be great. Thanks, *Laura Tarwater* *Tissue Bank Technician* *Harper Cancer Research Institute* *University of Notre Dame574-631-2562* *tarwate...@nd.edu <tarwate...@nd.edu>* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet