Hi, My experience with microarrays is that they are sometimes a bit complicated to work with depending on the way they are constructed and the platform they are used on. Often these slides are dipped in paraffin to help preserve them. When this happens they need considerably longer deparaffinization. They also sometimes are not floated on a waterbath to transfer them to the slide but by a tape transfer process. The tape often interferes in IHC staining and especially on Ventana platforms that make use of the liquid coverslips. If you can try to get microarrays that are floated like regular sections, make sure you give them a good long soak in xylene, and perhaps increase the volume per slide of the antibodies.
Best of luck, Amos On Wed, Jan 27, 2016 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Message: 5 > Date: Wed, 27 Jan 2016 14:15:05 +0000 > From: John Shelley <jshel...@sbpdiscovery.org> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Tissue Arrays > Message-ID: > <c54f513da7da7547b37103a4b74bdae903e4b...@carrera.ln.burnham.org> > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > > I have purchased some arrays from a company in the past and had some very > varying results. I prefer not to mention the company and so my request is > if those of you who do not have the luxury of having tissue at your > disposal to make your own tissue arrays from whom are you buying them from. > Once purchased have you used them on IHC platforms like Ventana > Benchmark/Ultra or Leica Bond3? Were your results consistent across the > slide? > > I would like to add that I am looking for neuroblastoma tumor arrays. Any > help will be greatly appreciated. Thanks in advance! > > Kind Regards! > > John J Shelley > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet