What kind of processor are you using? On Tue, Jan 26, 2016 at 1:36 PM, Jennifer MacDonald via Histonet < histonet@lists.utsouthwestern.edu> wrote:
> There are a couple of things that it might be. > 1. Uneven deparaffinization before staining. > 2. Water in your last reagents/paraffin on the processor. > > > > From: mohamed abd el razik via Histonet > <histonet@lists.utsouthwestern.edu> > To: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Date: 01/26/2016 11:33 AM > Subject: [Histonet] unsolved proplem > > > > Dear allI have submitted a proplem about reprocessing tissue blocks that > have patches of stained areas and unstained pale yellow areas (H&E > stain)!!!, unfortently the proplem didn't solved yet and i have tried all > possible solutions, increasing processing time and ordered new chemicals > from other soruces and changed the paraplast company but still have the > proplem !!!! > i'm using 70% alc 2hr - 80% alc 1hr - 90% alc 45mins- absolut 1 and 2 each > for 30 mins then Benzen 1 and 2 each for 25 mins then clearing by methyl > benzoat 2hs at least then paraffin1+2 (Maccormick melting degree > 56-58)each 90 mins for mice kidney, liver and tests > the previous protocol was very satisfactory to us and we have tried to > stain older sections processed befor the emerging proplem and have a nice > stain by current H&E stain to ensure that the proplem is out our staining > step. > any suggestions please !! > Mohamed > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
