Hi James, Nuclear bubbling artifact is most commonly seen in formalin fixed epithelial cells, and GI biopsies are among those samples that are particularly susceptible to it. It has been linked to inadequate fixation and also to heating of slides prior to staining without complete air-drying of the tissues. I would recommend cutting the block again, air drying the slides for a time before using heat to melt the wax prior to H&E stain and see if the artifact persists.
http://www.cap.org/apps/docs/proficiency_testing/nuclear_bubbling.pdf Best wishes, Teri Johnson ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
