Hi Charles,
A couple things to check on:
1. The first concern I would have is your EA stain. Poor EA staining
will give too much orange staining and pale other counterstains (making
them look aged). What brand are you using? Have you changed brands?
Is your EA close to the expiration date? Is the bulk stored with light
exposure? I think fixing your EA will fix all the other problems.
2. The other question I have regards your alcohol. Have you changed
types/brands? Pap staining is very delicate and the different alcohols
used can make a big difference.
Beth Cox, HTL/SCT(ASCP)QIHC
------------------------------
Message: 3 Date: Thu, 4 Feb 2016 09:50:18 -0500
From: Charles Riley <cri...@dpspa.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAP stain quality
Message-ID:
<CAAQhB11_GcJXjHd-BLQqDfZuTDDPMoOGCCd1g=d-uehpwrq...@mail.gmail.com>
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Not sure if anyone out the would know the answer to this. We are having
an issue with our PAP stained slides appearing too orange and look aged.
If you have any idea for causes I appreciate any help
-- Charles Riley HT(ASCP)CM
Histopathology Coordinator/ Mohs
Doctors Pathology Services, Dover DE
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