Deanne,
I purchase 5 liter cubes from Mercedes Medical.
They can provide test results on each batch of water and fax to you.
I did have a CLIA inspector advise to document daily on the water as is
written on the cube for clarity of water, no sediments or signs of
contamination.
Think 5 Liters is about $12.50 and I use a little less than two a month.
Brent Adams – BS, LPN, HT
www.acadianagastro.com
Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA 70503
tel: (337) 269-1126
fax: (337) 269-1476
________________________________________
From: histonet-requ...@lists.utsouthwestern.edu
<histonet-requ...@lists.utsouthwestern.edu>
Sent: Tuesday, February 16, 2016 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 147, Issue 15
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Today's Topics:
1. (CLRW) - Clinical Laboratory Reagent Water (Knutson, Deanne)
2. Tissue cassette baskets for VIP 6 (Vickroy, James)
3. MICROTOME KNIFE SHARPENING (Hannen, Valerie)
4. Re: (CLRW) - Clinical Laboratory Reagent Water (Morken, Timothy)
5. Nuclear Bubbling (Vickroy, James)
6. Complementary webinar on post-processing scientific images,
Feb 24 @ 1PM EST (J. Sedgewick)
----------------------------------------------------------------------
Message: 1
Date: Mon, 15 Feb 2016 12:14:06 -0600
From: "Knutson, Deanne" <dknut...@primecare.org>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water
Message-ID:
<1e0e2b14c709174b8ac2be0ae7f76833a4d5970...@exchange2k7.staprimecare.org>
Content-Type: text/plain; charset="us-ascii"
Fellow Histonetters,
Our medical center will no longer be providing us with water for our laboratory
procedures.
I was wondering where other labs who need to purchase Clinical Laboratory
Reagent Water are doing so?
Thank you for your help!
Deanne Knutson
Supervisor
Anatomic Pathology
________________________________
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Message: 2
Date: Mon, 15 Feb 2016 19:12:06 +0000
From: "Vickroy, James" <jvick...@springfieldclinic.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Tissue cassette baskets for VIP 6
Message-ID:
<9b1a1501a800064397369bd8072e6bca06502...@e2k10db.springfieldclinic.com>
Content-Type: text/plain; charset="us-ascii"
Anybody have an idea where we can get a used cassette basket that will fit in
the VIP 6? The cost of a new one is pretty high?
Jim
Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois 62703
Office: 217-528-7541, Ext. 15121
Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com>
This electronic message contains information from Springfield Clinic, LLP that
may be confidential, privileged, and/or sensitive. This information is intended
for the use of the individual(s) or entity(ies) named above. If you are not the
intended recipient, be aware that disclosure, copying, distribution, or action
taken on the contents of this information is strictly prohibited. If you have
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retrieval of this electronic message. Thank you.
------------------------------
Message: 3
Date: Tue, 16 Feb 2016 10:28:59 -0500
From: "Hannen, Valerie" <valerie.han...@parrishmed.com>
To: "Histonet@lists.utsouthwestern.edu"
<Histonet@lists.utsouthwestern.edu>
Subject: [Histonet] MICROTOME KNIFE SHARPENING
Message-ID: <450B7A81EDA0C54E97C53D60F00776C323546750BC@isexstore03>
Content-Type: text/plain; charset="us-ascii"
Hi all... Hoping you might be answer a couple of questions that I have. 1)
Does anyone use a microtome knife sharpening kit made by Pathco?
If so, 2) How well does it sharpen the blades? 3) Is there any issue on
attaching the abrasive sheets to the honing plate? 4) Do the abrasive sheets
tend to "move"
during the sharpening process, that would cause the blade not be on them but on
the plate instead?
I currently am using coarse and fine abrasive liquids along with the honing
plate... but the coarse, fine and honing liquids are becoming quite expensive
and hard to come by.
Any and all replies are welcomed.
Thank you in advance,
Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com>
www.parrishmed.com
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Message: 4
Date: Tue, 16 Feb 2016 16:05:12 +0000
From: "Morken, Timothy" <timothy.mor...@ucsf.edu>
To: "Knutson, Deanne" <dknut...@primecare.org>
Cc: Histonet <histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] (CLRW) - Clinical Laboratory Reagent Water
Message-ID:
<761e2b5697f795489c8710bcc72141ff6fd17...@ex07.net.ucsf.edu>
Content-Type: text/plain; charset="us-ascii"
Deanne,
If you just need it for a few critical reagents You can get type 1 water by the
pint or the gallon from Fisher (NERL Type 1 water). It's probably overkill for
most histology procedures, but convenient if you need it. Note that the expiry
clock of 30 days starts when you open the bottle.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-----Original Message-----
From: Knutson, Deanne via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, February 15, 2016 10:14 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water
Fellow Histonetters,
Our medical center will no longer be providing us with water for our laboratory
procedures.
I was wondering where other labs who need to purchase Clinical Laboratory
Reagent Water are doing so?
Thank you for your help!
Deanne Knutson
Supervisor
Anatomic Pathology
________________________________
This email may include confidential and privileged information. If this is not
intended for your use, please destroy immediately and contact the sender of the
message.
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privileged. If you are not the intended recipient, notify the sender at once
and delete this message completely from your information system. Further use,
disclosure, or copying of information contained in this email is not
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privilege or other confidentiality protections.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Tue, 16 Feb 2016 17:10:40 +0000
From: "Vickroy, James" <jvick...@springfieldclinic.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Nuclear Bubbling
Message-ID:
<9b1a1501a800064397369bd8072e6bca06503...@e2k10db.springfieldclinic.com>
Content-Type: text/plain; charset="us-ascii"
Struggling to find an answer. We do a lot of GI biopsies in our lab.
Sometimes they look wonderful without any nuclear bubbling, other times the
bubbling is pretty intense. Since nuclear bubbling is often attributed to
incomplete fixation we of course have investigated the fixation times. I do
not find that the problem is fixation. In fact some of the biopsies end up
fixing for 48 hrs before processing. (weekend). There was a suggestion last
week or so that there might be water trapped under the slides after cutting and
before staining. I really thought that this might be the issue however I'm
not sure at this point. Extra drying seems to help but sometimes slides side
by side are so variable, one with bubbles and one without. I also don't
believe the problem is in the processing schedule since the problem has shown
up on both a rapid and a normal schedule. (therefore longer dehydration,
clearing, etc.)
I am wondering if anyone else has worked with this issue. Here are my
questions:
1. Could it be something that is happening with the tissue before it
gets to the lab? Usually a delay if fixation causes other artifacts but not
bubbling. Could it be heat from the GI procedure?
2. We do use blue sponges for our biopsies. I know some say get rid of
the sponges but has anyone seen this problem caused by usage of sponges?
3. What about the heat stage in our Prisma stainer?
I am really getting frustrated. Pathologists never complain however I would
rather all of the tissue did not have the "nuclear bubbling". Again we only
do biopsies so I really don't think the standard old " not enough time in
formalin" is the issue. I have even wondered about variables such as we use
recycled formalin, recycled Clearite III.
Any suggestions?
Jim
Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois 62703
Office: 217-528-7541, Ext. 15121
Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com>
This electronic message contains information from Springfield Clinic, LLP that
may be confidential, privileged, and/or sensitive. This information is intended
for the use of the individual(s) or entity(ies) named above. If you are not the
intended recipient, be aware that disclosure, copying, distribution, or action
taken on the contents of this information is strictly prohibited. If you have
received this electronic message in error, please notify the sender
immediately, by electronic mail, so that arrangements may be made for the
retrieval of this electronic message. Thank you.
------------------------------
Message: 6
Date: Tue, 16 Feb 2016 11:31:27 -0600
From: "J. Sedgewick" <jerrysedgew...@gmail.com>
To: <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Complementary webinar on post-processing
scientific images, Feb 24 @ 1PM EST
Message-ID: <1557B579CB2A478B884FD20B9E5064D6@sedge>
Content-Type: text/plain; format=flowed; charset="UTF-8";
reply-type=original
Hello All,
Do you post-process your scientific images? Typical tools for common
adjustments include Photoshop and ImageJ, but they aren?t the best answer.
You are invited to attend a complementary webinar on Feb 24 at 1:00PM EST ?
?Best Practices for Post-Processing of Scientific Images?
Reserve your webinar seat now at
https://attendee.gotowebinar.com/register/1379379490730827010
I'll be giving this webinar. Also, a similar presentation will be given for
the histology WOW 2016 with Dr. Michael Linden at the University of
Minnesota on March 25.
Best,
Jerry Sedgewick
------------------------------
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