I wonder if streptavidin Alexa (495 or 488) can be use to bind to biotinlyted 
primary. So no worry about species issue? May need to do biotin blocking first 
to get ride of endogenic biotin I guess. 

Dorothy Hu


Sent from my iPad

> On Feb 20, 2016, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Double stain IHC question

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