Hi Histonetters, I have been trying to get CD24(SN3b)( cat# MA5-11833) from Thermo Fisher to stain paraffin sections with no success. I tried antigen retrieval with citra and cc1. I have tried ovarian tumor tissue, tonsil, colon tumor tissue and did not get any staining. So I called Thermo Fisher and they said to try the antibody without antigen retrieval and I did that and still did not get any staining. So I tried protease 2 digestion for 8 min. and still did not get any staining. I have been trying the antibody at a 1:50 dilution for 1 to 2 hours. Does anyone have any suggestions on how to make this antibody work?
Elaine Dooley U of FL 352-265-0111 ext 72117 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
