Happy Friday, Histonetters! I have a puzzle that I could really use your expert knowledge on this morning. One of our pathologists has a decalcified FFPE mouse bone embedded longitudinally and has found some bacteria located in the growth plate. The bacteria has been confirmed with fluorescence, but she really wants to be able to show the morphology of the tissue with the bacteria. So far, H&E, gram, Brown & Hopps stain the growth plate so dark that you can't make out the bacteria. My suggestions were to try a thinner section and adjust the timing of the H&E (haven't had a chance to do that yet), but I wanted to throw the question out to you all to see if anyone has tried other stains or techniques for this type of issue.
I'd really appreciate the help! Thanks, Anna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
