Hey Charles, Why are the first 2 steps of formalin that long? If you make sure your tissue is well fixated, then there is no need for such long fixation in the machine. You could use step 2 for just washing the formalin out, so for only like 5 minutes. You could use that time, to add to other chemicals.
Hope it works for you. Maarten Werdler 2016-03-22 9:26 GMT-06:00 Charles Riley via Histonet < histonet@lists.utsouthwestern.edu>: > We are having issues with our tissue processing. We use the thermo shandon > excelsior processor. All steps have agitation and under vacuum. Our routine > process is as follows > > 1. Formalin 1hr > 2. Formalin 1hr > 3. 75% alcohol 30 mins > 4.85% alcohol 30 mins > 5. 95% alcohol 30 mins > 6.95% alcohol 30 mins > 7. 100% alcohol 30 mins > 8. 100% alcohol 30 mins > 9 xylene 30 mins > 10. xylene 30 mins > 11 xylene 30 mins > 12. Wax for 30 mins > 13. Wax 30 mins > 14. Wax 30 minutes. > > Can anyone give any suggestions for altering this process to work better? I > know the process should probably be longer however medical director does > not want to delay turn around time. If possible keep the process to under 9 > hours. If this isn't conducive to consistent processing please explain why > so I can show my superiors to give myself some leverage to change things > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > Doctors Pathology Services, Dover DE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet