You might find these articles to be useful Tracy, R. E., & Walia, P. (2002). A method to fix lipids for staining fat embolism in paraffin sections. Histopathology, 41(1), 75-79.
Turello, R., Snyder, D., & Hartman, H. A. (1984). A modification the osmium tetroxide post-fixation technique for the demonstration of extracellular lipid in paraffin-embedded tissue sections. Journal of Histotechnology, 7(2), 75-77. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Hobbs, Carl via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Saturday, 26 March 2016 5:30 AM To: histonet Subject: Re: [Histonet] Porcessing FFPE tissue without alcohol?? Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) Processing to Pwax as usual. Basically, you will see lipids as black ( oxidised osmium) That's the only way to demonstrate solvent- soluble lipids, using Pwax processing. Sure, there are caveats but, in the main...it will be Ok, imho. I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
