What i would do, melt the blocks you made. 1. Put them back in paraffin for one hour 2. Put them back in another paraffin or one hour 3. Put them in xylene for one hour 4. Put them in another xylene for one hour 5. Put them in another xylene for one hour 6. Put them back in paraffin for one hour 7. Put them back in paraffin for one hour.
It takes some time. The reason why it was dificult to cut is because the alcohol and paraffin dont mix, there is a possibility that the parafin was not well enough impregnated in the tissue. With the above solution, you can restore that. Maarten UNAM neurobiologia, histología Mexico 2016-04-07 4:43 GMT-05:00 Charles Riley via Histonet < histonet@lists.utsouthwestern.edu>: > Hello all, > > We had an issue with our processor the other night. Someone accidentally > put 100% alochol into the last xylene station. The tissue sections were > difficult to cut and or were unreadable. What is the best way to reprocess > them? They are mostly small GI biopsies and only two larger specimens > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
