Good to know that I am not the only on that think Rene is negative to others.
Hey Rene it's not just one person that thinks your a dirt bag.
________________________________________
From: histonet-requ...@lists.utsouthwestern.edu
<histonet-requ...@lists.utsouthwestern.edu>
Sent: Tuesday, May 3, 2016 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 150, Issue 2
Send Histonet mailing list submissions to
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To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. No More Blog Posts -- Over and Out! (Lester Raff MD)
2. IHC positive controls (Cindy Bulmer)
3. Re: No More Blog Posts -- Over and Out! (Rene J Buesa)
4. Fwd: (Linda Margraf)
5. Re: No More Blog Posts -- Over and Out! (Manfre, Philip)
6. Re: IHC positive controls (Morken, Timothy)
7. Re: No More Blog Posts -- Over and Out! (Boyd, Debbie M)
8. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO)
9. Re: No More Blog Posts -- Over and Out! (Caroline Miller)
10. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO)
11. Re: Fwd: (Rene J Buesa)
12. HHV-8 RTU (Delray Beach Pathology Kari Simeone)
13. PAS/Decal Question (Marcum, Pamela A)
----------------------------------------------------------------------
Message: 1
Date: Mon, 2 May 2016 17:39:28 +0000
From: Lester Raff MD <lr...@uropartners.com>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] No More Blog Posts -- Over and Out!
Message-ID:
<6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local>
Content-Type: text/plain; charset="us-ascii"
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the
best if I no longer post links to my blog, lab related or otherwise. Of course
the blogs go on, and if anyone is interested in being added to my mailing list
for future notifications, just drop me a line at
les.r...@post.com<mailto:les.r...@post.com> The mailing list is never used
for any purpose other than announcing a new blog post. Be sure to let me know
you are from the Histonet list!
I will continue to participate in any histology/pathologist discussions here as
I have for many years.
Cheers,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
------------------------------
Message: 2
Date: Mon, 2 May 2016 13:42:36 -0400
From: Cindy Bulmer <cjbulmer2...@aol.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls
Message-ID: <15472908bba-2169-b...@webprd-a99.mail.aol.com>
Content-Type: text/plain; charset=utf-8
Hello Histoland,
I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?
1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for
1 hr.
then put slides in refrigerator for future use.
2) Cut (serial sections, stain the last slide for bugs) NO oven time and put
slides
directly in refrigerator for future use.
3) Cut "fresh" every time they order the Ab.
Thank you,
Cindy
Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX
------------------------------
Message: 3
Date: Mon, 2 May 2016 18:22:59 +0000 (UTC)
From: Rene J Buesa <rjbu...@yahoo.com>
To: Lester Raff MD <lr...@uropartners.com>,
"'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID:
<1946825210.5059374.1462213379630.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren?
On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the
best if I no longer post links to my blog, lab related or otherwise.? Of course
the blogs go on, and if anyone is interested in being added to my mailing list
for future notifications, just drop me a line at
les.r...@post.com<mailto:les.r...@post.com>? The mailing list is never used for
any purpose other than announcing a new blog post. Be sure to let me know you
are from the Histonet list!
I will continue to participate in any histology/pathologist discussions here as
I have for many years.
Cheers,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Mon, 2 May 2016 13:37:35 -0500
From: Linda Margraf <lindamarg...@gmail.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd:
Message-ID: <31cf79c7-84c3-45b8-af7b-20151c22f...@gmail.com>
Content-Type: text/plain; charset=us-ascii
> From: Cindy Bulmer <cjbulmer2...@aol.com>
> Date: May 2, 2016 at 12:15:22 PM CDT
> To: histonet-ow...@lists.utsouthwestern.edu
>
> Hello Histoland,
>
> I need some advice, I have a PT block that is positive with Spirochetes.
> What would be the best way to use this block as a positive control?
>
> 1) Cut (serial sections, stain the last slide for bugs) and oven time (60)
> for 1 hr.
> then put slides in refrigerator for future use.
> 2) Cut (serial sections, stain the last slide for bugs) NO oven time and
> put slides
> directly in refrigerator for future use.
> 3) Cut "fresh" every time they order the Ab.
>
> Thank you,
> Cindy
> Cynthia Bulmer HT(ASCP),QIHC
> IHC Supervisor, CTPL
> Waco, TX
------------------------------
Message: 5
Date: Mon, 2 May 2016 14:38:32 -0400
From: "Manfre, Philip" <philip_man...@merck.com>
To: Rene J Buesa <rjbu...@yahoo.com>
Cc: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID:
<558a4571351d0c42bd923f403f4198c40111d88d1...@usctmxp51014.merck.com>
Content-Type: text/plain; charset="utf-8"
Delete button broken, huh. I suppose it was necessary to attack a colleague to
feel puffed up about yourself. Bully for you!
-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:23 PM
To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren?
On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the
best if I no longer post links to my blog, lab related or otherwise.? Of course
the blogs go on, and if anyone is interested in being added to my mailing list
for future notifications, just drop me a line at
les.r...@post.com<mailto:les.r...@post.com>? The mailing list is never used for
any purpose other than announcing a new blog post. Be sure to let me know you
are from the Histonet list!
I will continue to participate in any histology/pathologist discussions here as
I have for many years.
Cheers,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
------------------------------
Message: 6
Date: Mon, 2 May 2016 18:47:08 +0000
From: "Morken, Timothy" <timothy.mor...@ucsf.edu>
To: Cindy Bulmer <cjbulmer2...@aol.com>
Cc: Histonet <histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] IHC positive controls
Message-ID:
<761e2b5697f795489c8710bcc72141ff6fd47...@ex07.net.ucsf.edu>
Content-Type: text/plain; charset="us-ascii"
Cynthia, best practice is to keep in the block and cut as needed. Second is to
cut and dry at room temp and not melt, then store. Don't cut, melt and store.
The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect
the antigens better if they are fully isolated from air, which paraffin does,
but once cut, they are exposed. That happens in the fridge as well and I'm not
sure refrigeration helps much. I'm guessing that you don't do a lot of these,
so you don't want to cut a lot of slide and have them sitting around for months
waiting for use.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-----Original Message-----
From: Cindy Bulmer via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls
Hello Histoland,
I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?
1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for
1 hr.
then put slides in refrigerator for future use.
2) Cut (serial sections, stain the last slide for bugs) NO oven time and put
slides
directly in refrigerator for future use.
3) Cut "fresh" every time they order the Ab.
Thank you,
Cindy
Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Mon, 2 May 2016 19:08:22 +0000
From: "Boyd, Debbie M" <dkb...@chs.net>
To: "Manfre, Philip" <philip_man...@merck.com>
Cc: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID:
<7eafe982e328304da6ce2b677bb76246ab4b7...@tn001wexmbx014.us.chs.net>
Content-Type: text/plain; charset="utf-8"
I just feel if you didn't care for the subject/blog (doesn't pertain to you,
you don't have any exposure to it etc. ) you could have deleted it without
being so mean spirited. I have in the past ignored the mean spirited sparring,
but this was a bit much.
Whatever happened to kindness?
Just my two cents....
Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical
Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 |
FAX 804-765-6058
________________________________________
From: Manfre, Philip via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:38 PM
To: Rene J Buesa
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL] Re: [Histonet] No More Blog Posts -- Over and Out!
Delete button broken, huh. I suppose it was necessary to attack a colleague to
feel puffed up about yourself. Bully for you!
-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:23 PM
To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren?
On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the
best if I no longer post links to my blog, lab related or otherwise. Of course
the blogs go on, and if anyone is interested in being added to my mailing list
for future notifications, just drop me a line at
les.r...@post.com<mailto:les.r...@post.com> The mailing list is never used for
any purpose other than announcing a new blog post. Be sure to let me know you
are from the Histonet list!
I will continue to participate in any histology/pathologist discussions here as
I have for many years.
Cheers,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--------------------------------------------------------------------------
Disclaimer: This electronic message may contain information that is
Proprietary, Confidential, or legally privileged or protected. It is intended
only for the use of the individual(s) and entity named in the message. If you
are not an intended recipient of this message, please notify the sender
immediately and delete the material from your computer. Do not deliver,
distribute or copy this message and do not disclose its contents or take any
action in reliance on the information it contains.
------------------------------
Message: 8
Date: Mon, 2 May 2016 12:10:44 -0700
From: WILLIAM DESALVO <wdesalvo....@outlook.com>
To: "Manfre, Philip" <philip_man...@merck.com>, Rene J Buesa
<rjbu...@yahoo.com>
Cc: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID: <col402-eas17105428354e8afe011831082...@phx.gbl>
Content-Type: text/plain; charset="utf-8"
Your response is WAY out of line. Keep it professional.
Sent from my Windows Phone
________________________________
From: Manfre, Philip via Histonet<mailto:histonet@lists.utsouthwestern.edu>
Sent: ?5/?2/?2016 12:08 PM
To: Rene J Buesa<mailto:rjbu...@yahoo.com>
Cc:
'histonet@lists.utsouthwestern.edu'<mailto:histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Delete button broken, huh. I suppose it was necessary to attack a colleague to
feel puffed up about yourself. Bully for you!
-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:23 PM
To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren?
On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the
best if I no longer post links to my blog, lab related or otherwise. Of course
the blogs go on, and if anyone is interested in being added to my mailing list
for future notifications, just drop me a line at
les.r...@post.com<mailto:les.r...@post.com>? The mailing list is never used for
any purpose other than announcing a new blog post. Be sure to let me know you
are from the Histonet list!
I will continue to participate in any histology/pathologist discussions here as
I have for many years.
Cheers,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Mon, 2 May 2016 14:49:25 -0700
From: Caroline Miller <mi...@3scan.com>
To: Lester Raff MD <lr...@uropartners.com>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID:
<CAFTHRQNbrcnN4jyGQypUYEFPrPdyPVjSjJQC=2w4+orwtth...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Thank you Lester, I really appreciate your decision, and I look forward to
reading your histology-related histonet posts.
yours,
mills
On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> To My Lab Colleagues:
>
> As my intent has never been to sow discontent or rancor, I think it is for
> the best if I no longer post links to my blog, lab related or otherwise.
> Of course the blogs go on, and if anyone is interested in being added to my
> mailing list for future notifications, just drop me a line at
> les.r...@post.com<mailto:les.r...@post.com> The mailing list is never
> used for any purpose other than announcing a new blog post. Be sure to let
> me know you are from the Histonet list!
>
> I will continue to participate in any histology/pathologist discussions
> here as I have for many years.
>
> Cheers,
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
------------------------------
Message: 10
Date: Mon, 2 May 2016 15:49:09 -0700
From: WILLIAM DESALVO <wdesalvo....@outlook.com>
To: STEVEN PINHEIRO <spinhe...@lumc.edu>
Cc: <histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID: <col129-w27de73aed699c88244aa9582...@phx.gbl>
Content-Type: text/plain; charset="windows-1256"
Another personal e-mail from you that is unwanted. I am feeling like I have my
own stalker. You are one of the reasons that individuals will not post or
comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu)
chose you to represent them. I request that you no longer contact me and make
personal attacks. I know nothing of you and you certainly do not know anything
about me. If you have something to say, post to the listserve, not me.
> From: spinhe...@lumc.edu
> To: wdesalvo....@outlook.com
> Subject: RE: [Histonet] No More Blog Posts -- Over and Out!
> Date: Mon, 2 May 2016 20:15:38 +0000
>
> As is yours. Just can't keep that dictatorial aspect out of your charming
> consultant two cents worth.
>
> Steve Pinheiro
>
>
> -----Original Message-----
> From: WILLIAM DESALVO via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Monday, May 02, 2016 2:11 PM
> To: Manfre, Philip; Rene J Buesa
> Cc: 'histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
>
> Your response is WAY out of line. Keep it professional.
>
> Sent from my Windows Phone
> ________________________________
> From: Manfre, Philip via Histonet<mailto:histonet@lists.utsouthwestern.edu>
> Sent: ?5/?2/?2016 12:08 PM
> To: Rene J Buesa<mailto:rjbu...@yahoo.com>
> Cc:
> 'histonet@lists.utsouthwestern.edu'<mailto:histonet@lists.utsouthwestern.edu>
> Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
>
> Delete button broken, huh. I suppose it was necessary to attack a colleague
> to feel puffed up about yourself. Bully for you!
>
> -----Original Message-----
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Monday, May 02, 2016 2:23 PM
> To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
>
> Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren?
>
> On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet
> <histonet@lists.utsouthwestern.edu> wrote:
>
>
> To My Lab Colleagues:
>
> As my intent has never been to sow discontent or rancor, I think it is for
> the best if I no longer post links to my blog, lab related or otherwise. Of
> course the blogs go on, and if anyone is interested in being added to my
> mailing list for future notifications, just drop me a line at
> les.r...@post.com<mailto:les.r...@post.com> The mailing list is never used
> for any purpose other than announcing a new blog post. Be sure to let me know
> you are from the Histonet list!
>
> I will continue to participate in any histology/pathologist discussions here
> as I have for many years.
>
> Cheers,
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Notice: This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New
> Jersey, USA 07033), and/or its affiliates Direct contact information for
> affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely for
> the use of the individual or entity named on this message. If you are not the
> intended recipient, and have received this message in error, please notify us
> immediately by reply e-mail and then delete it from your system.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Confidentiality Notice:
> This e-mail, including any attachments is the property of Trinity Health and
> is intended for the sole use of the intended recipient(s). It may contain
> information that is privileged and confidential. Any unauthorized review,
> use, disclosure, or distribution is prohibited. If you are not the intended
> recipient, please delete this message, and reply to the sender regarding the
> error in a separate email.
------------------------------
Message: 11
Date: Tue, 3 May 2016 13:28:28 +0000 (UTC)
From: Rene J Buesa <rjbu...@yahoo.com>
To: Linda Margraf <lindamarg...@gmail.com>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Fwd:
Message-ID:
<1663136023.5351238.1462282108474.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
As I see it, the best solution is "1"Even more: if the piece of tissue is large
enough,?cut 1 section and stain ? select at least 2 (+) areas? divide the block
into 2 blocks each containing one of those 2 areas?and?by doing so?you would
have duplicated the number of possible (+) sections.Ren?
On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet
<histonet@lists.utsouthwestern.edu> wrote:
> From: Cindy Bulmer <cjbulmer2...@aol.com>
> Date: May 2, 2016 at 12:15:22 PM CDT
> To: histonet-ow...@lists.utsouthwestern.edu
>
> Hello Histoland,
>?
> I need some advice, I have a PT block that is positive with Spirochetes.
> What would be the best way to use this block as a positive control?
>?
> 1)? Cut (serial sections, stain the last slide for bugs) and oven time (60)
> for 1 hr.
>? ? ? then put slides in refrigerator for future use.
> 2)? Cut (serial sections, stain the last slide for bugs) NO oven time? and
> put slides
>? ? ? directly in refrigerator for future use.
> 3)? Cut "fresh" every time they order the Ab.
>?
> Thank you,
> Cindy
> Cynthia Bulmer HT(ASCP),QIHC
> IHC Supervisor, CTPL
> Waco, TX
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Message: 12
Date: Tue, 3 May 2016 16:29:18 +0000
From: Delray Beach Pathology Kari Simeone <ksime...@leavittmgt.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] HHV-8 RTU
Message-ID:
<43944b1dbaac2846b7b9d626b5f1233cc9185...@vm-email.leavittmgt.com>
Content-Type: text/plain; charset="iso-8859-1"
My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU.
Does anyone have a vendor they purchase from that I can research? Thanks so
much.
Kari M Simeone
Histology/Immunohistochemistry Specialist Supervisor
Alternate Laboratory Supervisor
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Message: 13
Date: Tue, 3 May 2016 16:39:16 +0000
From: "Marcum, Pamela A" <pamar...@uams.edu>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] PAS/Decal Question
Message-ID: <826681eae65c40b28de6f620b3207...@mail13m2n1.ad.uams.edu>
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We are still having issues with our PAS stain on decaled bone marrows. The
Pathologists in HemePath are seeing what they refer to as smudginess in cells
on some areas of the completed PAS slides. We have looked at everything and
cannot find where the issue is coming from at this point. We have done manual
staining for PAS, automated on the Leica stainer and on the Dako Artisan. All
methods show the same result for some slides. We can go for several days to a
week or more with no problem and then suddenly it is back and we have changed
nothing in the way we do the processing, embedding, sectioning,
deparaffinization and coverslipping. We do as many as 38 bone marrow cores a
night or as few as 8 and can find no correlation in the number we have to deal
with for a given period. All bone marrows drawn today must be completed by 8AM
tomorrow morning.
Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a
maximum of 7 hours +/-.
Grossed and placed in cassettes for 15 minute rinse in running DI Water
Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C
Rinsed in running DI water for 15 minutes
Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours
minimum to come off at 4:45AM.
If anyone knows of any literature on decal effects on PAS staining in bone
marrows please contact me. This has been going on for months and no matter
what we do manual staining, Leica adaptation for automated or Dako it is not
helping. Dako has been great with sending in technical experts repeatedly and
we cannot get this corrected.
Thanks,
Pam
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End of Histonet Digest, Vol 150, Issue 2
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