Valerie, We have 4 DAKOs. They are work-horses and are a very "open" platform. You can use all of your own reagents, nothing proprietary if you choose. This makes them good for research and optimizing your own protocols. They are also very consistent and reliable for conducting the same protocol repeatedly. Much cheaper to own and operate than a Ventana. This is not a slam on Ventana. Ventana strainers are quite amazing but way more expensive than a DAKO. The Ventana is partially open, or not open, depending on how you look at it. You can force it to use some of your own reagents but you always rely somewhat on Ventana reagents. These stainers are mostly designed to run the same set of protocols over and over again, consistently (e.g. hospital). They are also useful when used by technicians who want to "set it and forget it". If someone doesn't really know IHC, and the troubleshooting involved sometimes, then this is the unit for you if you can afford it. There is also the high pressure sales team that accompanies any interest in Ventana units. Don't underestimate their tenacity, especially if you agree to have a trial machine placed in your lab for testing. They will not want to remove it - an assumed sale, if you will. I am not on the payroll for anyone but Merck, lest anyone think I am trying to influence a sale one way or another. These are my personal experiences and opinions that I hope you will find useful.
Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -----Original Message----- From: Murphy, Valerie via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, May 04, 2016 11:52 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Automated IHC instrument Hello Histonetters, Our tissue core is interested in purchasing an IHC instrument. It would be used for the more routine assays such as ER/PR, Her2, Ki67 etc. Can anyone make a recommendation? Leica, Ventana, Dako? Thank you, Valerie Ratliff B.Sc HTL(ASCP) Research Assistant Department of Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: murp...@karmanos.org<https://webmail.karmanos.org/owa/redir.aspx?REF=3fWOArMaNAXm4nDSak3jq-gnqda3Gmrjo2JqVL-dzSB85MreM3TTCAFtYWlsdG86bXVycGh5dkBrYXJtYW5vcy5vcmc.> Better treatments. Better outcomes. ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet