Valerie,
        We have 4 DAKOs.  They are work-horses and are a very "open" platform.  
You can use all of your own reagents, nothing proprietary if you choose.  This 
makes them good for research and optimizing your own protocols.  They are also 
very consistent and reliable for conducting the same protocol repeatedly.  Much 
cheaper to own and operate than a Ventana.  This is not a slam on Ventana.
        Ventana strainers are quite amazing but way more expensive than a DAKO. 
 The Ventana is partially open, or not open, depending on how you look at it.  
You can force it to use some of your own reagents but you always rely somewhat 
on Ventana reagents.  These stainers are mostly designed to run the same set of 
protocols over and over again, consistently (e.g. hospital).  They are also 
useful when used by technicians who want to "set it and forget it".  If someone 
doesn't really know IHC, and the troubleshooting involved sometimes, then this 
is the unit for you if you can afford it.  There is also the high pressure 
sales team that accompanies any interest in Ventana units.  Don't underestimate 
their tenacity, especially if you agree to have a trial machine placed in your 
lab for testing.  They will not want to remove it - an assumed sale, if you 
will.
        I am not on the payroll for anyone but Merck, lest anyone think I am 
trying to influence a sale one way or another.  These are my personal 
experiences and opinions that I hope you will find useful.

Phil.

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_man...@merck.com



-----Original Message-----
From: Murphy, Valerie via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, May 04, 2016 11:52 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Automated IHC instrument

Hello Histonetters,
Our tissue core is interested in purchasing an IHC instrument. It would  be 
used for the more routine assays such as ER/PR, Her2, Ki67 etc.
Can anyone make a recommendation? Leica, Ventana, Dako?

Thank you,

Valerie Ratliff  B.Sc HTL(ASCP)
Research Assistant
Department of Oncology
Karmanos Cancer Institute
4100 John R
Detroit, MI 48201

Telephone: (313) 576 8282
Fax: (313) 576 8306
E-mail: 
murp...@karmanos.org<https://webmail.karmanos.org/owa/redir.aspx?REF=3fWOArMaNAXm4nDSak3jq-gnqda3Gmrjo2JqVL-dzSB85MreM3TTCAFtYWlsdG86bXVycGh5dkBrYXJtYW5vcy5vcmc.>

Better treatments. Better outcomes.


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