Hello, I'm currently using an ancient bottle of xylene based mounting medium and want to get something better that will serve multiple purposes.
In the past I had used entellan for DAB and routine histo stains (i.e. H&E) but then a student accidentally (recklessly?) used my Mowiol + n-propyl gallate mounting medium (that I use for fluorescence) for a PTAH stain and it is worked well, retaining the sharpness of the stain much longer than the entellan did. In the past I had used the Mowiol for DAB and that seemed to work well. I am now preparing to perform staining with AEC and Fastred that don't like the alcohol dehydration steps needed for a xylene mounting medium. Is there any reason why I can't simply use the Mowiol mounting medium for all my work? It isn't like the DAB is going to dissolve... I am using methyl green as a counter stain for my current work. The mowiol mounting medium is glycerol based ( http://cshprotocols.cshlp.org/content/2006/1/pdb.rec10255.full?text_only=true with n-propyl gallate instead of DABCO). Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
