On H&E, parietal cells are eosinophillic , due to abundant mitochondria.
Chief cells have basophillic cytoplasm on H&E, due to abundant rough
endoplasmic reticulum. Other than that, I'd think you'd want to use IHC.
Is here an antibody for intrinsic factor? Parietal cells secrete intrinsic
factor, chief cells do not.
Sincerely,
Jay A. Lundgren, M.S., HTL
(ASCP)
P.S. Abcam makes an anti-intrinsic factor Ab for IHC. Make sure to spell
my name right on the paper. ;P
On Wed, Jun 15, 2016 at 1:16 PM, Judi Ford via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hi everyone,
> I'm trying to differentiate parietal vs chief vs endocrine cells in the
> stomach. Any ideas on stains or antibodies I could use.
> Thanks,
> judi
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