Brett, I use the rabbit monoclonal CD3 from Thermo (it was a Lab Vision antibody) with great and consistent results!
The hardest part, actually getting the antibody to me....\jeez they have problems with orders sometimes... Here is the information for mine: CD3 (SP7) - cat #RM-9107-S1 I use it at 1:400, citrate retrieval, 1 hour primary incubation. I use the Rabbit on Rodent polymer from Biocare. Respectfully, ' Colleen Forster On Tue, Jun 21, 2016 at 10:53 AM, Connolly, Brett M via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Liz, > As long as it detects mouse T-cells it's OK. Unfortunately we have a > problem getting Dako products through our ordering system. > Brett > > -----Original Message----- > From: Elizabeth Chlipala [mailto:l...@premierlab.com] > Sent: Tuesday, June 21, 2016 11:20 AM > To: Connolly, Brett M; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] CD3 antibody for FFPE mouse tissue > > Brett > > Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking > for a CD3 that only detects mouse? > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > l...@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: Connolly, Brett M via Histonet [histonet@lists.utsouthwestern.edu] > Sent: Tuesday, June 21, 2016 7:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CD3 antibody for FFPE mouse tissue > > Hi all, > > Looking for recommendation for an anti-mouse CD3 antibody for FFPE > sections. > > I have some old Histonet messages referring to a Neomarkers RB-360 ab > which I found through Thermo, but I would like to hear about more recent > experiences/recommendations.... > > Thanks as always, > Brett > > Brett M. Connolly, Ph.D. > Prin. Scientist, > Translational Biomarkers - Imaging > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_conno...@merck.com<mailto:brett_conno...@merck.com> > T- 215-652-2501 > F- 215-993-6803 > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet