We add 50mls of 1% eosin to our first alcohol after the formalin.
1% EOSIN
FOR PROCESSING BIOPSIES
100mL ACETIC ACID
2g EOSIN
100mL DISTILLED WATER
Stir well!
Lisa Ryan
Histology
St James hospital
Dublin Ireland
________________________________________
From: histonet-requ...@lists.utsouthwestern.edu
[histonet-requ...@lists.utsouthwestern.edu]
Sent: 24 June 2016 17:00
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 151, Issue 22
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Today's Topics:
1. Marking Tissues with Eosin (Rebecca E. Ashley)
2. Re: Marking Tissues with Eosin (Mca Werdler)
3. Re: Shandon Varistain Gemini Slide Stainer (Teri Johnson)
4. Re: Marking Tissues with Eosin (Jamal Rowaihi)
5. Re: Shandon Varistain Gemini Slide Stainer (Paula Keene Pierce)
6. Re: Marking Tissues with Eosin (Cindy Bird)
7. Re: Marking Tissues with Eosin (Cynthia Robinson)
----------------------------------------------------------------------
Message: 1
Date: Thu, 23 Jun 2016 17:04:19 +0000
From: "Rebecca E. Ashley" <p...@uwyo.edu>
To: "Histonet@lists.utsouthwestern.edu"
<Histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Marking Tissues with Eosin
Message-ID:
<blupr05mb1874458c42d648382309fb96db...@blupr05mb1874.namprd05.prod.outlook.com>
Content-Type: text/plain; charset="us-ascii"
I had a biopsy today that was nearly impossible to see on the sponges during
embedding or in the block. I've heard mention of marking these with eosin to
make them easier to see. Has anyone done this? Or do you use some other type
of marking dye for this purpose?
Thanks for your input!
Rebecca
Rebecca Ashley
Histotechnologist
Wyoming State Vet Lab
1174 Snowy Range Rd.
Laramie, WY 82070
Phone: 307-766-9946
Fax: 307-721-2051
------------------------------
Message: 2
Date: Thu, 23 Jun 2016 12:49:37 -0500
From: Mca Werdler <mwerd...@gmail.com>
To: "Rebecca E. Ashley" <p...@uwyo.edu>
Cc: "Histonet@lists.utsouthwestern.edu"
<Histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Marking Tissues with Eosin
Message-ID:
<CAM7pV2mh7bt2T7npM5p=vfhV487=1kg3r2vcfl_dtvqf0dr...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Dear Rebecca,
Yes this is possible. just don't use a too strong concentration. The eosin
should give a slight pink color on the tissue after processing and after
embedding.
Good luck,
Maarten
2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
histonet@lists.utsouthwestern.edu>:
> I had a biopsy today that was nearly impossible to see on the sponges
> during embedding or in the block. I've heard mention of marking these with
> eosin to make them easier to see. Has anyone done this? Or do you use
> some other type of marking dye for this purpose?
> Thanks for your input!
> Rebecca
>
> Rebecca Ashley
> Histotechnologist
> Wyoming State Vet Lab
> 1174 Snowy Range Rd.
> Laramie, WY 82070
> Phone: 307-766-9946
> Fax: 307-721-2051
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 3
Date: Thu, 23 Jun 2016 19:04:44 +0000
From: Teri Johnson <tejohn...@genoptix.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer
Message-ID:
<d4d1b863c94a4982b41652bb37e19...@phuscb-sp37mb01.genoptix.org>
Content-Type: text/plain; charset=WINDOWS-1252
Hi Thomas,
Are you looking for true life expectancy or what is reported for depreciation?
In my experience, most tissue processors never die, but only need to be retired
due to lack of available support/parts or because a lab requires newer
technology.
Also in my experience, a "better" model comes out soon after I have purchased
one. So the life span is probably 20 years after you wished you had a different
one. :-)
Best wishes,
Teri
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA 92008
USA
Phone +1 760 516 5954
tejohn...@genoptix.com
www.genoptix.com
------------------------------
Hi All,
Does anyone know the life expectancy of the Gemini H&E stainer? My boss has
asked me this question because we are in the process of requesting for a new
one.
Thomas
Thomas Huynh BS, HT (ASCP)
Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM
5656 Kelly Street
Houston, Tx 77026
Lyndon B. Johnson General Hospital (LBJGH)
O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 |
thomas.hu...@harrishealth.org
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender
by return e-mail and delete this e-mail and any attachments from your computer
system.
To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability and
Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or
Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged. This e-mail may also be confidential and/or privileged under Texas
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If you are not the intended recipient, or any authorized representative of the
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------------------------------
Message: 4
Date: Thu, 23 Jun 2016 22:56:34 +0300
From: Jamal Rowaihi <j.rowa...@alborglaboratories.com>
To: Mca Werdler <mwerd...@gmail.com>, "Rebecca E. Ashley"
<p...@uwyo.edu>
Cc: ???? ??????? <j.rowa...@alborglaboratories.com>,
"Histonet@lists.utsouthwestern.edu"
<Histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Marking Tissues with Eosin
Message-ID: <pktvvaa0mu6cb5ppytq4gb9u.1466711448...@email.android.com>
Content-Type: text/plain; charset=utf-8
Hi?If you are using buffered Formaline so the Eosin color will not resist until
the end of tissue processing.I recommend to add small amount of stock Eosin to
the last alcohol in theTissue processor.?
Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent
from my cell phone
-------- Original message --------From: Mca Werdler via Histonet
<histonet@lists.utsouthwestern.edu> Date: 6/23/16 8:49 PM (GMT+03:00) To:
"Rebecca E. Ashley" <p...@uwyo.edu> Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Marking Tissues with Eosin
Dear Rebecca,
Yes this is possible. just don't use a too strong concentration. The eosin
should give a slight pink color on the tissue after processing and after
embedding.
Good luck,
Maarten
2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
histonet@lists.utsouthwestern.edu>:
> I had a biopsy today that was nearly impossible to see on the sponges
> during embedding or in the block.? I've heard mention of marking these with
> eosin to make them easier to see.? Has anyone done this?? Or do you use
> some other type of marking dye for this purpose?
> Thanks for your input!
> Rebecca
>
> Rebecca Ashley
> Histotechnologist
> Wyoming State Vet Lab
> 1174 Snowy Range Rd.
> Laramie, WY 82070
> Phone: 307-766-9946
> Fax: 307-721-2051
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Thu, 23 Jun 2016 21:02:49 +0000 (UTC)
From: Paula Keene Pierce <pa...@excaliburpathology.com>
To: Teri Johnson <tejohn...@genoptix.com>, Histonet
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer
Message-ID:
<994846485.545115.1466715770021.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
This is so true!
I had a Fisher Histomatic automated slide stainer that was manufactured in 1985
that I used until the building took a direct lightning strike JUST LAST YEAR!
My old VIP is still going!
?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N
Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX
405-759-7513www.excaliburpathology.com
From: Teri Johnson via Histonet <histonet@lists.utsouthwestern.edu>
To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>
Sent: Thursday, June 23, 2016 2:04 PM
Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer
Hi Thomas,
Are you looking for true life expectancy or what is reported for depreciation?
In my experience, most tissue processors never die, but only need to be retired
due to lack of available support/parts or because a lab requires newer
technology.
Also in my experience, a "better" model comes out soon after I have purchased
one. So the life span is probably 20 years after you wished you had a different
one. :-)
Best wishes,
Teri
Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA? 92008
USA
Phone +1 760 516 5954
tejohn...@genoptix.com
www.genoptix.com
------------------------------
Hi All,
Does anyone know the life expectancy of the Gemini H&E stainer? My boss has
asked me this question because we are in the process of requesting for a new
one.
Thomas
Thomas Huynh? BS, HT (ASCP)
Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM
5656 Kelly Street
Houston, Tx 77026
Lyndon B. Johnson General Hospital (LBJGH)
O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 |
thomas.hu...@harrishealth.org
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender
by return e-mail and delete this e-mail and any attachments from your computer
system.
To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability and
Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or
Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged.? This e-mail may also be confidential and/or privileged under Texas
law.? The e-mail is for the use of only the individual or entity named above.?
If you are not the intended recipient, or any authorized representative of the
intended recipient, you are hereby notified that any review, dissemination or
copying of this e-mail and its attachments is strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Thu, 23 Jun 2016 16:20:45 -0500
From: Cindy Bird <cb...@amli-denton.com>
To: Mca Werdler <mwerd...@gmail.com>
Cc: "Rebecca E. Ashley" <p...@uwyo.edu>,
"Histonet@lists.utsouthwestern.edu"
<Histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Marking Tissues with Eosin
Message-ID: <defa943f-8f9a-4a22-80c1-29d4f9ace...@amli-denton.com>
Content-Type: text/plain; charset="us-ascii"
We place a small drop of concentrate straight on tissue.
Sent from my iPhone
> On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet
> <histonet@lists.utsouthwestern.edu> wrote:
>
> Dear Rebecca,
>
> Yes this is possible. just don't use a too strong concentration. The eosin
> should give a slight pink color on the tissue after processing and after
> embedding.
>
> Good luck,
>
> Maarten
>
> 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
> histonet@lists.utsouthwestern.edu>:
>
>> I had a biopsy today that was nearly impossible to see on the sponges
>> during embedding or in the block. I've heard mention of marking these with
>> eosin to make them easier to see. Has anyone done this? Or do you use
>> some other type of marking dye for this purpose?
>> Thanks for your input!
>> Rebecca
>>
>> Rebecca Ashley
>> Histotechnologist
>> Wyoming State Vet Lab
>> 1174 Snowy Range Rd.
>> Laramie, WY 82070
>> Phone: 307-766-9946
>> Fax: 307-721-2051
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Fri, 24 Jun 2016 13:19:43 +0000
From: Cynthia Robinson <robin...@mercyhealth.com>
To: Cindy Bird <cb...@amli-denton.com>, Mca Werdler
<mwerd...@gmail.com>
Cc: "Histonet@lists.utsouthwestern.edu"
<Histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Marking Tissues with Eosin
Message-ID:
<4ee642d353925d4d96cb95e12427dbae56dd4...@nodcmstmbx06.no.trinity-health.org>
Content-Type: text/plain; charset="us-ascii"
We use safranin at the grossing station and it is a dark pink at embedding.
Works really well in our hands. Added plus is no fluorescent issues that you
can have with eosin.
Cindi
________________________________________
From: Cindy Bird via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, June 23, 2016 4:20 PM
To: Mca Werdler
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Marking Tissues with Eosin
We place a small drop of concentrate straight on tissue.
Sent from my iPhone
> On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet
> <histonet@lists.utsouthwestern.edu> wrote:
>
> Dear Rebecca,
>
> Yes this is possible. just don't use a too strong concentration. The eosin
> should give a slight pink color on the tissue after processing and after
> embedding.
>
> Good luck,
>
> Maarten
>
> 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
> histonet@lists.utsouthwestern.edu>:
>
>> I had a biopsy today that was nearly impossible to see on the sponges
>> during embedding or in the block. I've heard mention of marking these with
>> eosin to make them easier to see. Has anyone done this? Or do you use
>> some other type of marking dye for this purpose?
>> Thanks for your input!
>> Rebecca
>>
>> Rebecca Ashley
>> Histotechnologist
>> Wyoming State Vet Lab
>> 1174 Snowy Range Rd.
>> Laramie, WY 82070
>> Phone: 307-766-9946
>> Fax: 307-721-2051
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Subject: Digest Footer
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------------------------------
End of Histonet Digest, Vol 151, Issue 22
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