Hi Jim/Histonet world, This was a mistake and was not supposed to be posted. Histonet has already been emailed. Thank you! :)
Message: 1 Date: Thu, 18 Aug 2016 13:23:34 -0400 From: Jim Anzaldi <janza...@medequipsource.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Histonet Digest, Vol 153, Issue 15 Message-ID: <2C7597ABF5467247BE159D763E27F839016714B99787@PROMETHEUS. medequip.local> Content-Type: text/plain; charset="us-ascii" Vendor is soliciting sales item #1 *Corey Hubbard * Sales Account Manager / Marketing Admin 248.215.5390 ext 0022 *RANKIN* 14515 Mackey Road Holly, MI 48442 www.rankinbiomed.com facebook.com/rankinbiomed twitter.com/rankinbiomed.com *John 8:12* Jesus spoke to them, saying "I am the light of the world; he who follows me will not walk in darkness, but will have the light of life. On Fri, Aug 19, 2016 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Histonet Digest, Vol 153, Issue 15 (Jim Anzaldi) > 2. Re: Gallocyanin (Bob Richmond) > 3. Re: Gallocyanin - protocol (Elizabeth Chlipala) > 4. Re: Gallocyanine (Bob Richmond) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 18 Aug 2016 13:23:34 -0400 > From: Jim Anzaldi <janza...@medequipsource.com> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Histonet Digest, Vol 153, Issue 15 > Message-ID: > <2C7597ABF5467247BE159D763E27F839016714B99787@PROMETHEUS. > medequip.local> > > Content-Type: text/plain; charset="us-ascii" > > Vendor is soliciting sales item #1 > > -----Original Message----- > From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-request@ > lists.utsouthwestern.edu] > Sent: Thursday, August 18, 2016 1:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 153, Issue 15 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than > "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. update (Corey Hubbard) > 2. Re: Gallocyanine (Bob Richmond) > 3. Re: Gallocyanine (Elizabeth Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 17 Aug 2016 14:15:29 -0400 > From: Corey Hubbard <co...@rankinbiomed.com> > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] update > Message-ID: > <CAKSgtkf81Hv=jwsnFJqmYqwOmJe515QZ1uBq3RdcQBs6V+fS3g@mail. > gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Hi Eva, > > I wanted to see how things are going for you. Please let me know if you > come across any equipment needs. We specialize in histology and pathology > equipment and service. > > We are also always looking to purchase equipment - broken, used, > collecting dust. etc. > > Do you have a vendor you use currently for equipment? > > > > *Corey Hubbard * > Sales Account Manager / Marketing Admin > 248.215.5390 ext 0022 > > *RANKIN* > 14515 Mackey Road > Holly, MI 48442 > www.rankinbiomed.com > facebook.com/rankinbiomed > twitter.com/rankinbiomed.com > > *John 8:12* Jesus spoke to them, saying "I am the light of the world; he > who follows me will not walk in darkness, but will have the light of life. > > > ------------------------------ > > Message: 2 > Date: Wed, 17 Aug 2016 19:58:38 -0400 > From: Bob Richmond <rsrichm...@gmail.com> > To: "Histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Gallocyanine > Message-ID: > <CAOKsRH6Mi0ff3xC3NqgVifbTrUPhfM0EaAOxRcE0okhEvPF=6Q@mail. > gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Eva Permaul at Georgetown University asks: > > >>We just had a request for Gallocyanine staining. Does anyone do this? > >>Can > you share your protocol? Where do you buy your reagents?<< > > Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat > similar to celestin blue B. It's been used as a stain for Nissl substance. > Somebody at the AFIP was touting it as a hematoxylin substitute during the > Great Hematoxylin Shortage of around 1972. I think it was used as a chrome > alum lake for a nuclear stain. I've never seen it. You can get the dry dye > from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both > have formulas. But what does your client want to do with it? > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Message: 3 > Date: Thu, 18 Aug 2016 08:03:45 -0600 > From: Elizabeth Chlipala <l...@premierlab.com> > To: Bob Richmond <rsrichm...@gmail.com>, > "'histonet@lists.utsouthwestern.edu' > (histonet@lists.utsouthwestern.edu)" > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Gallocyanine > Message-ID: > <14E2C6176416974295479C64A11CB9AE02BED535B69A@SBS2K8. > premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > We have run this stain before, purchased the reagent from Sigma, it's > pretty easy to do there is a procedure in Bancroft and Gamble. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box > 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > l...@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Wednesday, August 17, 2016 5:59 PM > To: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Gallocyanine > > Eva Permaul at Georgetown University asks: > > >>We just had a request for Gallocyanine staining. Does anyone do this? > >>Can > you share your protocol? Where do you buy your reagents?<< > > Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat > similar to celestin blue B. It's been used as a stain for Nissl substance. > Somebody at the AFIP was touting it as a hematoxylin substitute during the > Great Hematoxylin Shortage of around 1972. I think it was used as a chrome > alum lake for a nuclear stain. I've never seen it. You can get the dry dye > from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both > have formulas. But what does your client want to do with it? > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 153, Issue 15 > ***************************************** > > > > > ------------------------------ > > Message: 2 > Date: Thu, 18 Aug 2016 20:01:51 -0400 > From: Bob Richmond <rsrichm...@gmail.com> > To: "Histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Gallocyanin > Message-ID: > <CAOKsRH7SOy458R_9CSM0XtKyKre_o4ayMLsANXW8HnEaQ3wDHw@mail. > gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Liz Chlipala at Premier Laboratory in Boulder CO writes: > > >>We have run this stain [gallocyanin] before, purchased the reagent from > Sigma, it's pretty easy to do there is a procedure in Bancroft and > Gamble.<< > > What stain? What mordant (chrome, aluminum, none)? To stain what? > > I think the person originally posting needs to extract this information > from the person who wants the stain done. > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Message: 3 > Date: Fri, 19 Aug 2016 08:24:06 -0600 > From: Elizabeth Chlipala <l...@premierlab.com> > To: Bob Richmond <rsrichm...@gmail.com>, > "'histonet@lists.utsouthwestern.edu' > (histonet@lists.utsouthwestern.edu)" > <histonet@lists.utsouthwestern.edu>, "e...@georgetown.edu" > <e...@georgetown.edu> > Subject: Re: [Histonet] Gallocyanin - protocol > Message-ID: > <14E2C6176416974295479C64A11CB9AE02BED535B6BD@SBS2K8. > premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > We used the stain to stain nissl substance in mouse lumbar spine. > > Purchased the dye from sigma - below is from the SDS Sheet > > Product name : Gallocyanine > Product Number : 124508 > Brand : Sigma-Aldrich > CAS-No. : 1562-85-2 > > The procedure in Bancroft-Gamble uses Chrom Alum - which is chromium > potassium sulfate as the mordant > > Chrom Alum - 5 gm > dH20 - 100 ml > Gallocyanine - 50 mg > > The chrome alum is dissolved in the distilled water, the gallocyanine is > added and the solution slowly heated until it boils. Boil for 5 minutes. > Cool to room temp adjust the volume back to 100 ml. Filter before use > > The stain is easy - place in staining solution for 18-48 hours, rinse in > water, dehydrate, clear and mount. B&G states results are blue but If I > remember correctly it turned out to be a bluish grey in color. I tried to > see if I had any images but I could not find any, we did this back in 2006 > so it was some time ago. > > If you search the histonet archives there is an older post of mine looking > for some help on this stain, there is a nice response back from Dr. Kiernan > explaining a bit more about the dye and staining. > > Liz > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > l...@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Thursday, August 18, 2016 6:02 PM > To: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Gallocyanin > > Liz Chlipala at Premier Laboratory in Boulder CO writes: > > >>We have run this stain [gallocyanin] before, purchased the reagent > >>from > Sigma, it's pretty easy to do there is a procedure in Bancroft and > Gamble.<< > > What stain? What mordant (chrome, aluminum, none)? To stain what? > > I think the person originally posting needs to extract this information > from the person who wants the stain done. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Fri, 19 Aug 2016 10:38:59 -0400 > From: Bob Richmond <rsrichm...@gmail.com> > To: Elizabeth Chlipala <l...@premierlab.com> > Cc: "'histonet@lists.utsouthwestern.edu' > (histonet@lists.utsouthwestern.edu)" > <histonet@lists.utsouthwestern.edu>, "e...@georgetown.edu" > <e...@georgetown.edu> > Subject: Re: [Histonet] Gallocyanine > Message-ID: > <CAOKsRH58yk3hYg6826HVJMco70tH8gyJgHj2yvgxsU-+Adwvzw@mail. > gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Here's John Kiernan's extremely informative review of gallocyanine (that's > how he says it's to be spelled) from the archives - I can't find a date on > it. Really tells you everything you need to know to do Nissl staining with > it. > > http://www.histosearch.com/histonet/May06/Re.Histonetgallocyaninest.html > > Remember to check on hazardous waste issues for chromium. > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 153, Issue 16 > ***************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet