We just installed a new Dako Link Autostainer and have started using the 
Herceptest Link kit.   We had been using two drop zones with our other, older 
instrument but that was applying only 100 uL in the 2 drop zones.   With this 
new program we  set it up to apply two zones of 200 uL but discovered that we 
will be cutting our test capability in half  (50 to about 25 slides).  How are 
other users handling this?   Do you use two drop zones, one on each end to 
cover the control and case material or do you have the reagents apply in one 
drop zone in the middle of the slide?  I am just concerned about the reagents 
spreading evenly across the slide.   What is everyone else's experience with 
this issue?

Thanks in advance for your help!

Martha Ward
Wake Forest Baptist Health

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