It's endogenous biotin, Tyrone. Make your own biotin blocking kit or buy RTU kit.
Also, endog. peroxidases are eliminated by 3% aq H2O2 ( saves methanol) for 10mins at RT Also, don't bother with permeabilisation.....you've done all that by processing the tissue to Pwax Also, use 10% Formalin. That's what I use for my zfish Pwax IHC All suggestions: imho. Have a look in Histonet image archives for IHC Pwax sections of z.fish Best wishes Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
