Hi there Histonetters! I am currently working through some issues of unprocessing tissue to then restain 'whole mount' with various stains, and then reprocess back to paraffin.
I am running into issues of tissue being brittle and scratchy tissue after reprocessing and sectioning. Can anyone offer any advice on kinder dewaxing procedures and / or additives to the unprocessing / processing fluids that might help in the resulting piece of tissue? I have currently been putting it through the cleaning cycle on the processor, so standard xylene then alcohol treatment. thanks in advance for your advice! mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
