RE: clip-on magnifiers
Try Ted Pella
Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email: cda...@che-east.org
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Subject: Histonet Digest, Vol 155, Issue 15
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Today's Topics:
1. Clip-On Magnifiers for Embedding (Lisa Hamilton)
2. Beta-Catenin (Algeo, Lacie A)
3. Picrosirius Red (Bax, N.A.M.)
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Message: 1
Date: Mon, 17 Oct 2016 14:22:26 -0500
From: "Lisa Hamilton" <lhamil...@wcplaboratories.com>
To: <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Clip-On Magnifiers for Embedding
Message-ID: <000901d228ab$cdacac90$690605b0$@com>
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In the past I have seen clip-on magnifiers that are used to embed tissue. Of
course now that I need one I cannot remember what catalog I saw them in! I
was hoping someone might
know where I can find them.
Thank You
-Lisa
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Message: 2
Date: Mon, 17 Oct 2016 19:53:28 +0000
From: "Algeo, Lacie A" <lacie.al...@providence.org>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Beta-Catenin
Message-ID:
<24c4b3c167e5694887ab594c7602ce3a03ce1...@wn35104.or.providence.org>
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Hi All,
What are people using for Beta-catenin control tissue? Is anyone using tissue
that is known specifically to have a mutation for the beta-catenin gene? If
so, is this something that is purchased?
Thanks,
Lacie
Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.al...@providence.org<mailto:lacie.al...@providence.org>
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Message: 3
Date: Tue, 18 Oct 2016 12:26:43 +0000
From: "Bax, N.A.M." <n.a.m....@tue.nl>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Picrosirius Red
Message-ID:
<67502d640fcb0641aacf2be17717060c325a9...@xserver30b.campus.tue.nl>
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Dear Histosearch,
I just became a member and I hope you can provide me with advice. I'm really
curious about the effect of not dissolving Direct Red 80 in 1.3% saturated
Picric Acid but in 0.9-1.1% alkalimetric Picric acid solution. The reason why
I'm curious is that I want to perform a staining on paraffin slides for Sirius
Red and in my current lab they have Picrosirius red dissolved in 0.9-1.1%
alkalimetric Picric acid, but the samples were I want to compare them to are
stained in Picrosirius red made with the standard protocol with saturated
Picric Acid.
I measured the pH of the Picrosirius Red made with 0.9-1.1% alkalimetric Picric
acid and this is ~pH 2.0 and all the Direct Red is dissolved, so can I assume
that the staining will be comparable to Picrosirius Red dissolved in saturated
Picric acid?
Thank you very much in advance.
Best Noortje
Noortje AM Bax, PhD | Laboratory for Cell and Tissue Engineering | Biomedical
Engineering | Eindhoven University of Technology, P.O. Box 513, Groene Loper,
Gebouw 15, Gemini-Zuid 4.108, 5600 MB Eindhoven, The Netherlands | T:
+31-40-2474830 | F: +31 40-2473744 |E-mail:
n.a.m....@tue.nl|<mailto:n.a.m....@tue.nl%7C>
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