Patrick, We've been studying BAP-1 too and do not have a good answer for a truly negative tissue, except for skeletal muscle, but decided against it. Our current multi control block contains: 1) lung ca, 2) tonsil, 3) colon ca, 4) LN, 5) ovary ca, 6) lung. Our clone specifically stains each tissue, either nuclear or cytoplasmic. My pathologists agonized over this for awhile, before they were happy with this mix.
Hugh-Hawaii ________________________________ Date: Tue, 8 Nov 2016 16:10:37 -0500 From: Patrick Laurie <foreig...@gmail.com> Subject: [Histonet] A suitable negative control for BAP1 Hello everyone, We recently brought BAP1 into our IHC menu, and we were eventually able to get a good stain that our pathologist was happy with. We are, however, looking for a good suitable negative control. Our pathologist is not certain and wanted me to ask the local community. Thanks, Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
