Hello, I was reading a book from the 60s on the anatomy of aging in man and animals and the author mentioned using a hematoxylin-eosin Y-Azure-II stain to show the lymphocytes. Some searching came up with the Maximow Method. The online protocol I found (for bone marrow): https://emsdiasum.com/microscopy/technical/datasheet/26252.aspx mentions the use of Zenker’s or Formalin.
In another old book, Putt's Manual of Histochemical Staining Methods, the authors says 10% fomalin, Helly's or Zenker's fluid for fixation. I am definitely not going to start using Zenker's (I get enough grief from the H&S officer about picric acid). I normally use Davidson's fixative to fix and decalsify my fish (formaldehyde, acetic acid + ethanol). Anyone know if this staining method is compatible with Davidson's fixative? Would this eosin-Y solution be suitable: http://www.sigmaaldrich.com/catalog/product/sigma/ht110316?lang=en®ion=US for preparing the Eosin-Y Azure-II working solution? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet