Dear all, We are doing some immunofluorescent stains that we want to pair with H&E slides. We are using fresh frozen sections for both (unfixed tissue, human diseased coronary arteries) but I am seeing very weak hematoxylin staining to the point where I have to dial back the eosin (3 min hematoxylin, 1 dip eosin).
I am using Gills 2 (newly purchased), I know its not necessary to filter it but we have been just in case using Whatman no. 5 filters. No differentiation due to the weak staining right now. I would appreciate any thoughts on why the staining might be so weak, we do a great deal of processing and imaging on the arteries (about 12 hrs @ 25C) prior to histo and I wonder if this has cause the tissue to decay? Thank you for your time, V -- ----------------------------------------------------------------------------------------- V Hou Bioengineering | Human Photonics Laboratory e: [email protected] | c: 206-999-3708 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
