> On 26 December 2016 at 22:15 taylor alan <aj.tay...@blueyonder.co.uk> wrote: > > > Hi Lauren > > With reference to your enquiry for the preparation of Wrights stain from > Wrights stain powder > > I have found the original method of preparing Wrights stain in one of my > favourite 'historical' pathology text books. Practical Bacteriology, Bloodwork > and Parasitology. Eighth Edition. Edited by Rear Admiral Professor E R Stitt. > USN. 1927. Who was the Director of the Naval Medical School in Washington and > the Phillipines. Many older technicians and medical staff who served time in > the Navy may remember this great text. > > Stitt details Wrights original method of preparing this stain, over a > paragraph, cutting tothe chase the resulting dried precipitate is scraped from > the filter paper for storage. The method for preparing a working solution is > to weigh out 300mg of stain powder and add to this in a flask 100ml of pure > (acetone free) methyl alcohol to dissolve.(All Romanowsky stains require this > same alcohol). > > Attached to this paragraph is an additional recommendation by Wright to > make a stock solution by weighing out 100mg of stain powder and dissolving > this in 60ml of methyl alcohol, as above. For use filter off 20ml and add to > the filtrate 5ml of pure methyl alcohol to make a working solution. > > I would be very happy to copy the whole paragraph for you, detailing the > lengthy preparation of this stain if you wish. If you have a very aged bottle > of this stain powder there is a very good chance that you will obtain better > results than from a stain prepared in more recent years. We have dry powder > stains that are more than 50 years old that give precise and beautiful > results, these are used sparingly and are very precious to us here in our > laboratory. > > Hoping you have success with your staining and the methodology detailed > above is helpful to you and your colleagues. > > Best Regards > > Alan Taylor BSc(Hons), FRMS. > > Microtechnical Services > > Exeter. UK. > > > > > > > On 22 December 2016 at 13:35 Lauren Sweeney via Histonet > > <histonet@lists.utsouthwestern.edu> wrote: > > > > > > Hello all in histoland- > > > > Does anyone out there have a recipe for preparing Wright's stain > > from powder? Our pathologist wants us to do Sheehan's modification Giemsa > > and we don't have any commercially prepared Wrights left but we do have an > > old bottle of Wright's powder. > > > > Happy Holidays! > > > > L > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >
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