Hello all, Does someone know what would cause dirty flakes of light purple that showed across the whole slide?
http://i.imgur.com/1nIXwAy.jpg Reagents are relatively fresh but the H&E stainer hadn't been used for a week over the holiday break. Is it surface film on the hematoxylin? I didn't notice anything in the container. We are using Mayer's, followed by differentiation with HCl acid alcohol, with 2-3 minute long wash steps in between. Thanks, Allan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
