I played around with it many years ago and found no difference except for the bluer tone. Simply substitute it for eosin Y ws gram for gram, and use the same technique.

Bryan Llewellyn



Bob Richmond via Histonet wrote:
Gudrun Lang (in Austria) asks:

I wonder how widespread the usage of eosin B is in H&E protocols. Is
there a
specific difference in application to Eosin Y?<<

Eosin B is closely related to the more commonly used eosin Y. As the
letters imply, it's Bluish rather than Yellowish.

I've never seen eosin B used in H&E staining protocols, but it certainly
has been.

Bob Richmond
Samurai Pathologist
Maryville, Tennessee
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