Hello all. Would there be any reason that slides stained with TUNEL (ISH) would not pick up the Mayers counterstain? We use it for IHC and it is fine. Mayers for 7 minutes and then ammonia water for 1 minute. We do that rather than rinse for 15 minutes and there are usually no issues. Could a reagent in the stain be causing it? DAPI is used... Bernice
Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
