I almost always have used SODIUM metabisulfite for any version of PAS. I am also familiar with procedures that go straight to tap water for the pot-Schiff's rinsing, though that was from a job in my past. I seem to remember that working fine.
Phil. Philip Manfre, HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP81-406 770 Sumneytown Pike West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -----Original Message----- From: Angela Lamberth via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, March 06, 2017 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium) In Carson’s 3rd edition, the reducing rinse following Schiff for PAS is 0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse is given as sodium metabisulfite (Carson pg 149). My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing rinse as sodium metabisulfite which makes sense to me since the Schiff is made with sodium metabisulfite. Is this a printing error in the Carson book? I appreciate any light anybody can shed on this. -- Angela Lamberth Histology Technician III Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet