When you fix the slide in 10% formalin you will have to perform AR.
TH ________________________________ From: histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> Sent: Wednesday, March 15, 2017 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 160, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Immunoperoxidase Protocol on Cytospin (Margaryan, Naira) 2. Re: Immunoperoxidase Protocol on Cytospin (Cartun, Richard) 3. Congo red and LED illumination problem? (Morken, Timothy) 4. Day Shift Histotech Job in Southern CA (Melissa Owens) 5. Bone Processing (MICHELLE BETH TITUNICK) 6. GSH Annual Histopalooza April 21st - April 23rd Legacy Lodge, Lake Lanier Islands, Georgia (Zimmerman, Billie) ---------------------------------------------------------------------- Message: 1 Date: Tue, 14 Mar 2017 18:50:45 +0000 From: "Margaryan, Naira" <nmargar...@luriechildrens.org> To: "histonet-boun...@lists.utsouthwestern.edu" <histonet-boun...@lists.utsouthwestern.edu> Cc: histonet-request <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Immunoperoxidase Protocol on Cytospin Message-ID: <34b2eda118548a4eb35d6e650345ba6469c06...@sv-ex08.childrensmemorial.org> Content-Type: text/plain; charset="utf-8" Dear histonetters: I never done ICC. A scientist brought me a slide with Cytospined cells on for IHC. I am going to fix with 10%NBF (it is only what I have now) then wash with TBST. May I skip AR step? May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer???? Thanks in advance, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) ------------------------------ Message: 2 Date: Tue, 14 Mar 2017 19:38:08 +0000 From: "Cartun, Richard" <richard.car...@hhchealth.org> To: "Margaryan, Naira" <nmargar...@luriechildrens.org> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Immunoperoxidase Protocol on Cytospin Message-ID: <9215bd4b0ba1b44d962a71c758b68d2e95401...@hhcexchmb03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" Since you only have 1 slide I would recommend following your regular protocol that you use for formalin-fixed, paraffin-embedded tissue. I would use heat retrieval if that's what you use for FFPE tissue. However, if you use enzyme digestion on FFPE tissue I would "not" digest your cytospin slide. If you don't get any immunoreactivity it could be a "False Negative" due to pre-analytical variables. I think it can be interesting to try experiments like this, but it's not the way to do IHC testing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Margaryan, Naira [mailto:nmargar...@luriechildrens.org] Sent: Tuesday, March 14, 2017 3:24 PM To: Cartun, Richard Subject: RE: Immunoperoxidase Protocol on Cytospin cytoplasmic staining for Nodal protein. thanks ________________________________________ From: Cartun, Richard [richard.car...@hhchealth.org] Sent: Tuesday, March 14, 2017 2:13 PM To: Margaryan, Naira; histonet-boun...@lists.utsouthwestern.edu Subject: RE: Immunoperoxidase Protocol on Cytospin What is the target? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Margaryan, Naira via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, March 14, 2017 2:51 PM To: histonet-boun...@lists.utsouthwestern.edu Cc: histonet-request Subject: [Histonet] Immunoperoxidase Protocol on Cytospin Importance: High Dear histonetters: I never done ICC. A scientist brought me a slide with Cytospined cells on for IHC. I am going to fix with 10%NBF (it is only what I have now) then wash with TBST. May I skip AR step? May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer???? Thanks in advance, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 3 Date: Tue, 14 Mar 2017 19:42:36 +0000 From: "Morken, Timothy" <timothy.mor...@ucsf.edu> To: Histonet <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Congo red and LED illumination problem? Message-ID: <761e2b5697f795489c8710bcc72141ff8e5c1...@ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Something new to me... A pathologist said he is getting reports that LED illumination on newer microscopes is leading to faint or even false negatives for congo red amyloid birefringence due to the difference in spectrum between LED and tungsten filament lamps. Has anyone else noticed this? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ------------------------------ Message: 4 Date: Wed, 15 Mar 2017 00:47:09 +0000 From: Melissa Owens <meli...@alliedsearchpartners.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Day Shift Histotech Job in Southern CA Message-ID: <d4ee0747.35ee7%meli...@alliedsearchpartners.com> Content-Type: text/plain; charset="us-ascii" Hello Histoland, I have a Day Shift Histotech opportunity in Southern California for full time permanent employment. Please contact me directly for more details. Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 ------------------------------ Message: 5 Date: Wed, 15 Mar 2017 09:58:08 -0400 From: "MICHELLE BETH TITUNICK" <mbt...@psu.edu> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone Processing Message-ID: <1489586288l.21430310l...@psu.edu> Content-Type: text/plain; charset=utf-8 Hello. Does anyone have a processing protocol for formalin fixed decalcified rat bones using an automated processor? I've been doing it manually with a vacuum oven and it takes 14-16 hours to complete. I don't have a vacuumed section on my automatic processor. Also any tips besides what I've read in the archives for immunohistochemistry? Thanks so much. Michelle B. Titunick PhD Candidate, Anatomy The Pennsylvania State University College of Medicine mbt...@psu.edu (973) 715-9831 ------------------------------ Message: 6 Date: Wed, 15 Mar 2017 15:21:13 +0000 From: "Zimmerman, Billie" <bzimm...@augusta.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] GSH Annual Histopalooza April 21st - April 23rd Legacy Lodge, Lake Lanier Islands, Georgia Message-ID: <mwhpr03mb2607cb3447f7389662f02637cb...@mwhpr03mb2607.namprd03.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" The Georgia Society for Histology Histopalooza is just around the corner!! Calling all procrastinators to register for the event. It's cheap, cheap, cheap compared to other CEU events. I spoke with Claudia Faulkenberry this morning. She will be speaking about the hot, hot antibody PDL1. (no, it ain't cheap ) It's on television commercials so the general public is asking about it. She will tell us the uses for this antibody as well as the various clones available. Companion diagnostics is the new buzz word for PDL1. Thank you Claudia. See you at Lake Lanier, Billie Zimmerman MT(ASCP)QIHC ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 160, Issue 15 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet