-----Original Message-----
From: Pablo Sánchez Quinteiro via Histonet
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, March 22, 2017 1:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sections falling off
Dear Histonetters,
I am in trouble with my paraffin sections -samples fixed in Bouin's liquid.
Pablo,
I think your sections are not completely dry, after placing them on
slides. Silanized slides can trap water between the sections and the slide,
almost appearing like a bulge. You may need to use the corner of a paper towel
to "poke" the paraffin section and drain off the excess water. Make sure the
sections look dry before starting the staining procedure. They should be
transparent (translucent), not opaque (milky white).
That's my guess
Phil.
Philip Manfre, HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP81-406
770 Sumneytown Pike
West Point, PA 19486
215-652-9750
215-993-0383 (fax)
philip_man...@merck.com
Just after the first steps: Two xylol baths and 100% ethanol most of
them are falling off. Slides are silanised.
I do not think it was an adhesion problem. What do you think about?
Embedding, cuttibg?
Thanks a lot in advance
Pablo
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