Tim and Tony, Why couldn't DAB be used on frozen sections in your example?
Allan On Mon, Apr 17, 2017 at 9:03 PM, Tony Henwood (SCHN) via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Bianca, > Well for most Pathology departments, Immunofluorescence (IF) is used for > Renal and Skin biopsies; looking for human Immunoglobulin (Igs) deposition > on basement membranes. The advantage here is not so much the fluorescence, > but that we use unfixed frozen sections. The buffer rinse before antibody > application, removes un-bound serum immunoglobulins, leaving any > pathological bound Igs for the IF antibody to bind to. This gives a clean > result. > > If one would do IF on formalin-fixed paraffin sections of renal or skin > biopsies, you would find heavy background due to the fixative cross-linking > serum Igs to tissue and cells (which would usually be removed by the buffer > rinse if unfixed frozen sections were used - see above). > > IF, apart from being a historic method, also does not suffer from > endogenous peroxidase that would need to be blocked if peroxidase was used > in place of fluorescence. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Thursday, 13 April 2017 11:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] help!! > > Hello! > I just need a help with a simple question...Is anyone can explain me what > is the purpose between performing immunohistochemistry and > Immunofluorescence? > Thanks :) > > Blanca Lopez > Histotech (ASCP) > UTSW Tissue Resource K1.210 > Simmons Comprehensive Cancer Center > UT Southwestern Medical Center > Telephone: 214-648-7598 > Email: blanca.lo...@utsouthwestern.edu > > > ________________________________ > > UT Southwestern > > > Medical Center > > > > The future of medicine, today. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet