Another reason that IHC is used instead of IF is with IHC, one preserves the ability to see tissue/cell morphology through Light Microscopy at the same time as the visual IHC label. Morphology is difficult to see with IF, with the exception of the fluorescein labeled area.
Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, 13 April 2017 11:10 PM Hello! I just need a help with a simple question...Is anyone can explain me what is the purpose between performing immunohistochemistry and Immunofluorescence? Thanks :) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: blanca.lo...@utsouthwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
