I would also check the fixation. Do the smears look air-dried. The larger nuclei, following air-drying do not concentrate the Hx as much as prompt alcohol fixation resulting in paler stained nuclei.
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, 20 April 2017 2:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology pap staining I have been put in charge of figuring out why our pap stains are light on the hematoxylin. Everything was filtered and used fresh as per usual using the same protocol we have used for the past two years. If anyone has any suggestions as to how to fix this problem I would greatly appreciate it. If you need more information please let me know and I will get it for you to help assist you in assisting me -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
