Hi Richard,
We use NCL-PARVO from Leica
Details as follows:
ANTIGEN NAME: Parvovirus B19
OTHER NAMES:
Clone: R92F6
Isotype: IgG1
DESCRIPTION: Mouse monoclonal to native parvovirus B19 purified from human
plasma. Human parvovirus B19 (VP1 and VP2 capsid proteins).
STORAGE CONDITIONS: 4-8oC undiluted
SUPPLIER: Leica NCL-PARVO
PROCEDURE:
Methodology: HRP-POLYMER Working Dilution: Bond: 1/100
Special Conditions: Citrate HIER required (ER1)
CONTROL TISSUE: Placenta containing Parvovirus
Inbuilt Controls
CLINICAL SIGNIFICANCE:
Diagnosis of parvovirus B19 infection in formalin-fixed, paraffin embedded
tissue can be made through the identification of the characteristic brick-red
intranuclear inclusions in normoblasts within the fetal circulation in
histological sections stained with HE. It has been found that
immunohistochemistry was the best detection method. It is highly specific and
sensitive, preserves the morphology and reveals a larger number of positive
cells than does HE with the advantage of showing cytoplasmic and nuclear
positivity, making it more reliable.
REFERENCES:
Li, J. J., Henwood, T., Van Hal, S., & Charlton, A. (2015). Parvovirus
infection: an immunohistochemical study using fetal and placental tissue.
Pediatric and Developmental Pathology, 18(1), 30-39.
Quemelo PRV, Lima DM, Fonseca BAL, Peres LC (2007) "Detection of parvovirus B19
infection in formalin-fixed and paraffin-embedded placenta and fetal tissues"
Rev. Inst. Med. trop. S. Paulo, 49(2): 103-107,
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, 24 May 2017 4:33 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC testing for Parvovirus (Human)
If you do this test, where do you get your antibody? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax
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