I have no experiences w butterflies, but the chitin in ticks, carpenter ants and spiders does not cut well. For insects and arthropods we post-fix in Carnoy's for 60 minutes Then soften with 10% KOH RT for 10-20 minutes then wash for 30-60minutes in water. Long processing cycle.
Steve A. McClain, MD On May 30, 2017, at 13:26, "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> wrote: >> Does anyone have any experience processing and paraffin embedding >> butterflies? >> >> Rhonda Gregoire, MLT >> Supervisor, Clinical Pathology >> Veterinary Diagnostic Services >> Manitoba Agriculture >> 545 University Crescent, Winnipeg, MB, R3T 5S6 >> rhonda.grego...@gov.mb.ca<mailto:rhonda.grego...@gov.mb.ca> >> T : 204-945-7641 F : 204-945-7646 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
