I would say it is highly dependent on the antibody and application you are using. We don't fix any of our frozen tissue and no antigen retrieval is necessary. However, whenever we receive specimens that have been through a full FFPE process then, yes, the same antibody requires some antigen retrieval.
Best, Kristyn > ---------- Forwarded message ---------- > From: "Nair, Indu" <[email protected]> > To: "[email protected]" <[email protected] > > > Cc: > Bcc: > Date: Tue, 20 Jun 2017 22:08:59 +0000 > Subject: [Histonet] frozen sections > > Hello Histoneters! > > question: Does frozen section need Antigen Retrieval? > > thank you, for the anticipated timely help! > > indu > > > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
