Hello Histonetters! I am currently working in a Mohs lab in Minnesota and during the winter time we have no issues with humidity but comes summer, my slides get washed out by the end of the day. I have tried everything I can think of but NOTHING works.
I have tried changing solutions throughout the day, keeping the stainer lid open, adding molecular sieve in my alcohols and xylene substitutes, and a dehumidifier. We have the mini Thermo linear stainer under an airfiltronix filter which adds to the humidity issue. If anybody out there has other solutions I haven't tried yet please send them my way! One more thing that I have heard a long time ago at a conference is that if you use xylene substitutes then you do not have to use a hood/any type of ventilation system. Does that sound correct to anyone? Is that statement true? I have worked in labs that coverslip straight from alcohol and a ventilation system is not required but I notice the staining is not optimal in the long run. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
