Hi Beth, We have found the same thing. You get a moderate staining (half of what you see with most B cell lymphomas) with clone 1EW (as supplied with the Leica Bond rtu). You will get similar results with clone 24 (38%: Adams, et al (2009). Clinical, phenotypic and genetic similarities and disparities between post-transplant and classical Hodgkin lymphomas with respect to therapeutic targets. Expert opinion on therapeutic targets, 13(10), 1137-1145.)
We have found that using the Rabbit monoclonal antibody to Pax-5 (Clone SP34), it seems that, though staining a similar proportion of B cell lymphomas, staining is absent from the Reed-Sternberg cells of Hodgin lymphoma. Our assessment is in its early stages so with further cases, it might be clearer. It seems that not all Pax-5 antibodies react the same Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Linda Margraf via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Saturday, 22 July 2017 7:00 AM To: histonet@lists.utsouthwestern.edu Cc: 'one...@wvumedicine.org' Subject: [Histonet] FW: PAX-5 Here is a message I am posting for Beth…. From: "O'neil, Beth" <one...@wvumedicine.org<mailto:one...@wvumedicine.org>> Date: July 21, 2017 at 2:31:24 PM CDT To: "histonet-ow...@lists.utsouthwestern.edu<mailto:histonet-ow...@lists.utsouthwestern.edu>" <histonet-ow...@lists.utsouthwestern.edu<mailto:histonet-ow...@lists.utsouthwestern.edu>> Subject: PAX-5 My pathologists are not happy with PAX-5 on the Ventana Ultra; it will occasionally show cytoplasmic staining of the Reed-Sternberg cells (supposed to be a nuclear stain). We use Ventana’s clone SP34. I have been trying to optimize with the Optiview kit but we are getting so much background staining and only sporadic staining of the R-S cells. I tried a different clone, Cell Marque EP156 which was really clean but would not stain the R-S cells at all, no matter what we did. My Ventana technical specialist is stumped as to why we’re having so much trouble. Does anyone else have the same problem or have any suggestions? Beth Ann O’Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor, Technical Specialist Lab: 304 – 293 – 6014 Office: 304 – 293 – 7629 one...@wvumedicine.org<mailto:one...@wvumedicine.org> ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet