Hello Jenniffer,

we store the slides with section at 4-8 °C for many month or even longer and we 
do not have any problems with ISH.

Best regards
Michael



> Am 28.08.2017 um 17:30 schrieb Jennifer Phinney via Histonet 
> <histonet@lists.utsouthwestern.edu>:
> 
> Hello Histonet,
> I am hoping someone can point me in the right direction to find some 
> references for how long FFPE slides intended for ISH can be kept before 
> staining.  I have a researcher who swears their slides have to be stained 
> within 24 hours of being cut, which does not sound correct to me.  I've used 
> ACD's RNAscope before and their protocol lists the timeframe as 3 months (so 
> this is a bit of a difference).
> 
> There's a lot of information about fixation times, but I've come up empty 
> handed for cut slides.
> 
> Thanks everyone!
> 
> Jennifer Phinney QIHCCM
> Project Coordinator
> Kansas State University
> Veterinary Diagnostic Lab
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> Histonet@lists.utsouthwestern.edu
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