We fixed our BM biopsies at least 24 hours, but no more than 72 h in 10% NBF. Wash with tap water 30 mins. Decal into 14% EDTA (2Na salt) buffered with NaOH up to 7.0 overnight or weekend and test endpoint by weight loss weight gain method (special thanks to Gayle Callis!). Wash in tap water 1 hour. Process with isopropanols (IPA), mixtures IPA and mineral oil), paraffin wax. Microtomy at 3 microns. Stain HE, Giemsa, HC, IHC. -- Maxim Peshkov, Russia, Taganrog. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
